The dried seeds of Benincasa hispida (THUNB.) COGN. on successive extraction was extracted with solvents of increasing polarity by Soxhlet apparatus.The petroleum ether, ethanolic and aqueous extracts of seeds of Benincasa hispida (THUNB.) COGN. (250 and 500 mg/kg body weight) and standard drug cystone (500 mg/kg body weight) were used.Ethylene glycol (EG) being a chief substitute for alcohol, EG is commonly used as anti freeze in cooling systems of automobiles, aircrafts and has wide industrial applications.Toxicity from EG is produced from the metabolites such as glycoaldehyde, glyoxalate and oxalate, producing wide spread tissue injury in the kidney. The Aqueous, Ethanolic and Petroleum ether seed extracts of Benincasa hispida (THUNB.) COGN. Were tested at 250 and 500mg/kg doses for Nephroprotective Activity. The effects of both ethanolic and petroleum ether extracts of Benincasa Hispida were evaluated according to the method of LIPSCHITZ. Frusemide was employed as a standard diuretic agent (100mg/kg).
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